EFFECT OF SORO OIL ON KERATINOCYTE PROLIFERATION BY IN VITRO AND IN VIVO METHODS
SORO Oil, studied in detail for its effect on keratinocyte proliferation suppression.
The study was carried out in The Department of Microbiology, The New College, Chennai.
For in vitro study, EPISKIN & EpiDerm model were used. The fast growing cell
line were maintained either cryo-preserved or in 12% CO2 chamber. The SORO oil was
prepared in various dilutions in RPMI 1640 medium.
Immunomodulatory property of SORO Oil
SORO Oil was studied for its immunomodulatory property both by in vitro and in vivo
techniques. The phagocytes were isolated from the peritoneal cavity of Balp C mice
after injecting with thyoglycolate. The phagocytes were washed and fixed on a flat
bottom ELISA plate and were maintained in RPMI 1640. The adhered phagocytes were
pre-treated with different dilutions of SORO Oil for 20 minutes. Later the cells
were infected with opsonized fungal cells of Candida albicans. The level of phagocytosis
and phagocytic killing (phagocytic Index, PI) were calculated by the following formula
PI = Test / contro l Similarly, SORO Oil was administered orally to mice after injecting
with SRBC (Sheep Red Blood Cells) and the level of antibody production was estimated
Immunomodultory studies with SORO Oil reveal that SORO Oil can significantly increase
both phagocyte and antibody mediated immunity.
% reduction of cell division after treated with SORO Oil atVarious concentrations
Dose dependant decrease in cell multiplication was observed in the in vitro study.
20mg/ml concentration of SORO Oil was very effective in retarding the cell division
to 90% level.
SORO Oil at 50 mg/ml and above produced necrosis in the cell line.
The kinetics of cell division in the case of treated vs. untreated cells was 2:8.
The nuclear spindle formation is arrested during the treatment of cells with SORO
Oil. This phenomenon was studied by mitosis inhibition assay using onion root tip
assay. SORO Oil arrest the cell division especially during the mid anaphase to metaphase
Clinical and experimental studies on the efficacy of SORO Oil a herbal preparation
The efficacy of SORO Oil was confirmed on 300 cases taken up for trials in the out
patient department of Central Research Institute, Siddha at Chennai, India between
1998 to2001 The results were published in the Monograph by Central Council for Research
in Ayurveda and Siddha (CCRAS), Ministry of Health and Family Welfare, Government
of India, New Delhi .
The results together with the follow-up study indicated SORO Oil proved to be an
ideal drug that provided marked relief from clinical symptoms, broke the cycle of
recurrence, minimizes extent of complications and prevents complications completely.
Long-term application did not invite or foresee any hazards, unlike steroidal formulations.
Papers presented in Domestic conferences by Dr. Gaurang Joshi
Dr. Gaurang Joshi and his associates have presented a number of research and clinical
study papers in Domestic and International forums.
30 patients were put to treatment with SORO Oil for a period of 12 weeks. In a majority
of the cases, the clinical symptoms started receding within the period of study.
SORO Oil was found to a great extent, an ideal drug for the treatment of Psoriasis.
It was found to be clinically effective, Non-toxic, cosmetically acceptable and
economic. The findings results of the above study done by Prof. Dr. J.M. Boopalraj,
Dr. Frederick Manuel et al. were presented at the 23rd National Conference of IADV&L
(Indian Association of Dermatologists, Venerologists and Leprologists) in 1998 at
Chennai and later published in the issue of "The Antiseptic" Medical Journal.
A paper titled " Treatment of psoriasis with Wrightia tinctoria leaf extract"
was presented by Dr. Frederick Manuel at the above conference in . The paper highlighted
that the extracts of the leaves of the plant are effective and inexpensive for the
treatment of psoriasis and that most patients had long remissions on follow-up.
The paper was presented on the basis on the basis of the trials done at the Kilpauk
Medical College, Chennai.
Use experience reports of practising Dermatologists. SORO Oil and SORO Ointment
have been drugs of choice for many practising Dermatologists in India and under
their care, more and more patients are benefiting. Their observations have been
encouraging. There are many more Dermatologists, General Physicians and practitioners
of alternative medicines who share the observation. A few are presented below: "An
ideal drug for the treatment of Psoriasis should have all the following characteristics-
Effectiveness, Relative non-toxicity, Cosmetic applicability, and Cheapness (economical)
. In my experience I have found that SORO Oil satisfies all these parameters to
a great extent" " The more chronic the lesion the more striking is the
improvement with the application of SORO Oil" "SORO Oil can be considered
as one of the drugs of choice for the treatment, control and overall management
of Psoriasis" " I have personally prescribed SORO Oil to some of my eligible
patients and I found the results encouraging. Most of my patients got good remission"
" I would like to thank Dr. Gaurang Joshi for introducing SORO Oil on scientific
lines in the treatment of Psoriasis and conclude that SORO Oil is really a breakthrough
in the management of all types of Psoriasis" "For the last few years I
have been trying SORO Oil for my patients and I find the results are very effective
in regard to several signs and symptoms affecting patients. Most of my patients
had no remissions for a long period. None of my patients complained of any side
effects" " SORO Oil is an ideal drug for psoriasis and has no side effects"
" I have been prescribing SORO Oil and SORO Ointment to my Psoriasis patients
and some cases of Seborrhic dermatitis with satisfactory results. SORO Ointment
gives good results for Pityriasis Versicolor." This also explains the modern
views where scientific community believes that there is no proven link between diet
and its effect on psoriatic conditions but still some patients experience remarkable
improvements in their psoriatic conditions with the control of some of the diets
and then the same diet control does not produce any effect on other patients and
yet the same diet control does not produce any significant effect on same patient
after some time. How can we explain this anomaly. Our special Dietary Guideline
which we provide to every psoriatic patient under our treatment is based upon above
Ayurvedic View and help every psoriatic patients in their early cure.
E. Noiesen, M.D. Munk, K. Larsen, M. Høyen and T. Agner. (2007) Use of
complementary and alternative treatment for allergic contact dermatitis. British
Journal of Dermatology 157:2, 301-305 Eran Ben-Arye, M. Frenkel, M. Ziv. (2004)
An Approach to Teaching Dermatologists About Complementary Medicine. The Journal
of Alternative and Complementary Medicine 10:5, 899 E. Ernst, MD, PhD, FRCP. (2003)
CAM in dermatology: Telling fact from fiction. International Journal of Dermatology
42:12, 979-980 Gottlieb, AB, Lifshitz, B, Fu, SM, Staiano-Coico, L, Wang, CY,
Carter, DM: Expression of HLA-DR molecules by keratinocytes and presence of Langerhans
cells in the dermal infiltrate of active psoriatic plaques. J Exp Med 1986 164:
1013-1028, Gottlieb, AB: Immunologic mechanisms in psoriasis. J Am Acad Dermatol
1988 18: 1376-1380, | Gottlieb, AB, Chang, CK, Posnett, DN, Fanelli, B, Tam, JP:
Detection of transforming growth factor alpha in normal, malignant, and hyperproliferative
human keratinocytes. J Exp Med 1988 167: 670-675, Grossman, RM, Krueger, J, Yourish,
D, Granelli-Piperno, A, Murphy, DP, May, LT, Kupper, TS, Sehgal, PB, Gottlieb, AB:
Interleukin-6 (IL-6) is expressed in high levels in psoriatic skin and stimulates
proliferation of cultured human keratinocytes. Proc Natl Acad Sci USA 1989 86: 6367-6371,
Krueger, GG: Psoriasis: current concepts of its etiology and pathogenesis. In: Dobson
RL (ed.). Year Book of Dermatology 1981, Year Book Medical Publishers, Chicago,
pp 33-70, Baker, BS, Swain, AF, Fry, L, Valdimarsson, H: Epidermal T lymphocytes
and HLA-DR expression in psoriasis. Br J Dermatol 1984 11: 555-564, Baadsgaard,
O, Tong, P, Hansen, E, Taylor, RS, Chan, L, Mannie, A, Wantzin, GL, Voorhees, JJ,
Fox, D, Cooper, KD: Lesional psoriasis T cell clones express UM4D4, the surface
component of an antigen independent T cell activation pathway (abstr). J Invest
Dermatol 1988 90: 545, Kaplan, G, Witmer, MD, Nath, I, Steinman, RM, Laal, S,
Prasad, HK, Sarno, EN, Elvers, U, Cohn, ZA: Influence of delayed immune reactions
on human epidermal keratinocyres. Proc Natl Acad Sci USA 1986 183: 3469-3473,
Nathan, CF, Kaplan, G, Levis, WR, Nusrat, A, Wirmer, MD, Sherwin, SA, Job, CK, Horowitz,
CR, Steinman, RM, Cohn, ZA: Local and systemic effects of intradermal recombinant
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For More Details contact
B.A.M.S.(Gold Medalist) Ayurveda Immunologist,
Panchakarma Physician, Skin Specialist,
2,Paras Society, Nirmala Convent Road,